Nanoparticle Containing sRAGE for Diabetic Wound Healing

We present a novel therapeutic peptide delivery system to improve the healing of diabetic chronic wounds. We have developed fusion proteins of elastin-like polypeptides (ELPs) and the soluble receptor for advanced glycation end product (sRAGE) that self-assemble at physiological temperatures. These nanoparticles can be easily purified from a bacterial culture system by inverse temperature cycles, thus enabling rapid and inexpensive purification. In the present study, the fusion proteins were expressed in E.coli and purified by inverse transition temperature cycling. The results indicated that unlike other biopeptide fusion proteins previously reported, sRAGE-ELP nanoparticles are mostly expressed in bacteria as inclusion bodies.